Principles and Practice of Agarose change Electrophoresis Title: Principles and Practice of Agarose Gel Electrophorsis Objectives: To detect the size , shape and charge of the each tinge solution. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flaskfulful the buffer solution was prepared. using the equation to make enough solution for the intire lab score the equation had to be multiplyed by five. The contents of this equation were added to the 250ml flask and swirled to as distrubute it contents.
Then a mark was placed on the outside of the flask to indecate the level of the solution before heating. The flask opening had perafilm placed over it so that there was flyspeck to no evaporation. The solution was then placed in the microwave and heated. The solution was then heated for one min and swriled for evenly disolved agarose. The agarose was then cooled, so that it was not to hot and the plate would crack. some(a) water w...If you want to get a full essay, holy order it on our website: Ordercustompaper.com
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